Welcome to sRNAPrimerDB

sRNAPrimerDB is a comprehensive web primer or probe design tool specifically for small non-coding RNAs (sncRNAs), such as microRNA (miRNA, 20-25 nts), PIWI-interacting RNA (piRNA, 24-32 nts), short interfering RNA (siRNA, 20-25 nts), etc.

sRNAPrimerDB allows users to design several types of primers including generic or specific reverse transcription primers (Linear poly(T) RT primers, Linear S-poly(T) RT primers, stem-loop RT primers, stem-loop poly(A) RT primers, stem-loop poly(T) RT primers, Stem-loop S-poly(T) RT primers), specific PCR forward and reverse primer pairs, double hairpin probe, hybridization oligos for nine methods, such as:

Detailed experimental procedures for each method can be found in the protocol page. In addition, sRNAPrimerDB is also a primers bank, which contains tens of thousands of primer pairs for detecting miRNAs and piRNAs. Primers for sncRNAs that have been experimentally validated are encouraged to submit an online application form by visiting our web site at http://123.57.239.141 or www.srnaprimerdb.com.

If you make use of the data presented here, please cite the following article in addition to the primary data sources:

Shengsong Xie^†, Qin Zhu^†, Wubin Qu^†, Zhong Xu, Xiangdong Liu, Xinyun Li, Shijun Li, Wubin Ma, Yiliang Miao, Lisheng Zhang, Xiaoyong Du, Wuzi Dong, Haiwei Li, Changzhi Zhao, Yunlong Wang, Yaping Fang* and Shuhong Zhao*. sRNAPrimerDB: Comprehensive primer design and search web service for small non-coding RNAs. Bioinformatics, 2018, Doi:10.1093/bioinformatics/bty852.