CRISPR/Cas System (Cas9, Cas12, Cas13, Cas14, etc.)
Step 1 : Enter the query target sequences in FASTA format (example)
Step 2 : Enter the genome sequences in FASTA format (example)
Upload the reference genome in FASTA format (Note: the maximum allowed size for the uploaded genome is 8M)
Step 3 : Describe the PAM and sgRNA requirements
Custom PAM
Orientation
Length of sgRNA
Step 4 : Choose an off-target setting
No. of mismatches:
Step 5 : Click the button below to scan for sgRNA