sgRNAcas9-AI

Design specific sgRNA (On-target & Off-target)

CRISPR/Cas System (Cas9, Cas12, Cas13, Cas14, etc.)

Step 1 : Enter the query target sequences in FASTA format (example)

Or upload your input file: No file selected

Step 2 : Enter the genome sequences in FASTA format (example)

Step 2 : upload the custom reference genome: No file selected

Upload the reference genome in FASTA format (Note: the maximum allowed size for the uploaded genome is 8M)

Step 3 : Describe the PAM and sgRNA requirements

Custom PAM

Orientation

Length of sgRNA

Step 4 : Choose an off-target setting

Yes

No

No. of mismatches:

0M

1M

2M

3M

4M

5M

Step 5 : Click the button below to scan for sgRNA

Job ID: