Welcome to sgRNAcas9-AI

sgRNAcas9-AI is a web application that allows a user to upload target sequences, set specifications according to spCas9 and its variants (eSpCas9(1.1), HypaCas9, evoCas9, SpCas9-VRQR, Sniper-Cas9, SpCas9-HF1, SpCas9-NG and xCas9), and recieve target sgRNA candidates.
Using large-scale test data, we developed 9 deep-learning-based computational models that accurately predict the activity of Cas9 and its variants at any target sequence in human and animal cells.Optimal candidates are suggested through consideration of predicted target efficiency and off-target effect.

How to Cite:
1. Xie S, Shen B, Zhang C, Huang X, Zhang Y. sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One. 2014 Jun 23;9(6):e100448. doi: 10.1371/journal.pone.0100448.
2. Nie et al., sgRNAcas9-AI: a program for prediction of CRISPR/Cas9 and its variant sgRNA activity using deep learning, In prepare, 2022.